Electron Microscopic Immunogold Labeling of Bromodeoxyuridine (BrdU) in Routine Electron Microscopy.

Immunogold Labeling of Cultured Cells and Virus Particles for Electron Microscopy and Cryo-Electron Microscopy Applications

Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) were first realized in the early 1980s [1] and mid-1990s [2, 3]. Since then both methods have quickly become a powerful tools in structural biology. The major advantage is that they allow for the acquisition of images of biological specimens persevered in their native state after they are plunge-frozen onto carbon-coated ...

Immunogold labelling for scanning electron microscopy.

Scanning electron microscopes are useful biological tools that can be used to image the surface of whole organisms, tissues, cells, cellular components and macromolecules. Processes and structures that exist at surfaces can be imaged in pseudo or real 3D at magnifications of anything from about x10 to x1,000,000. Therefore a whole multicellular organism, such as a fly, or a single protein embed...

Immunogold labeling of amelogenin in developing porcine enamel revealed by field emission scanning electron microscopy.

The present study describes a method using immunohistochemical labeling in combination with high-resolution imaging (field emission scanning electron microscopy) to investigate the spatial localization of amelogenins on apatite crystallites in developing porcine enamel. Cross-sections of developing enamel tissue from freeze-fractured pig third molar were treated with antiserum against recombina...

Electron Microscopy of Fowl Pox Virus

Examination of Lung Parenchyma by Electron Microscopy

In this paper many of the principal struc­ture of the Moral Lung were studied with the help of Electron Microscopy. Detailed descrip­tion was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumo­cytes.  Acknowledgements  I am grateful to the Reza Shah Pahlavi Foundation for its generou...